Repurposing of pharmaceutical drugs by high-throughput approach for antihypertensive activity as inhibitors of angiotensin-converting enzyme (ACE) using HPLC-ESI-MS/MS method

Angiotensin-converting enzyme (ACE) plays an important role in regulating blood pressure the body by converting angiotensin-I into angiotensin-II. It is basic component of Renin angiotensin aldosterone system (RAAS), imbalance RAAS may leads to many cardiovascular and renal diseases. There are marketed available drugs for inhibition ACE, but prolonged use some cause progressive side effects. Repurposing existing can be a way find new inhibitors ACE. In this study, high-throughput sensitive method HPLC-ESI-QqQ-MS with good reproducibility (%RSD < 9.98) linearity (R2 = 0.999) was used investigate 77 commercial their inhibitory potential as antihypertensive drugs. Among these drugs, 41 were found active 36 them showed moderate lowest IC50 272 µM. This study that different pharmaceutical also ACE inhibitor after necessary clinical trials or validation.